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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pLTRpoly

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4273
Notes
Expression shuttle vector with promoters for in vitro RNA synthesis. (personal communication) Consists of the following sequences: nt 1-51 from pGEM7Zf(+) (reverse of 28-78), 52-70 from pUC19 (reverse of 428-446), 71-1090 from pSV2neo (reverse of 3050-4069), 1091-3474 from pSP72 (88-2462 and 1-9), (personal communication) and 3475-4273 from pMP-1 (with 7505-8297 from MoMuLV). (personal communication) The order of the major features in this plasmid is: HindIII site of MCS (nt = 1) - SV40 splice acceptor - SV40 late polyadenylation signal - T7 promoter - bla - SP6 promoter - XhoI site - MoMuLV LTR. (personal communication) Note that the T7 and SP6 promoters are not immediately adjacent to the cloning sites. (personal communication) When cotransfected with pSV2-neo (ATCC 37149) into NIH 3T3 cells, 70-80% of G418-resistant colonies expressed high levels of appropriately sized mRNA. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; PstI--4.3; XhoI--3.6; 0.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: mouse, E.coli HB101, E.coli DH5alpha, vertebrate cells, E.coli. Related vectors: pGEM-7Zf+, pUC19, pSV2neo, pSP72, pMP-1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77109
GenBank
X60280
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence