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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pMAC5-8

Information

Plasmid Type
Unspecified
Cloning Method
Unknown
Size
3803
Notes
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Corrected 20-DEC-1986 by J. Thierack, MPI Biochemie, Martinsried Corrected 10-JUL-1987 by P. Stanssens and F. Pfeiffer Vector for multiple rounds of oligonucleotide-directed construction of mutations. One derivative (pMA5) confers ampicillin resistance, the other (pMC5) confers resistance to chloramphenicol. Using specific oligonucleotides, resistance is shifted together with site-directed mutagenesis between these two resistance modes. The PstI site in the polylinker region is not unique. Presence of CAA at pos 2811-13 was shown by DNA sequencing. It is unknown, if this also applies to the parent sequence (pBR325)! Hosts: E.coli. Related vectors: PF1, pBR325, Poly8, EcoTrp. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank
L08930, A10238, A06419, A08598
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence