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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: YEp352

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5181
Notes
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. [1] The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, & pUC18 includes nt 2769-285. [2] Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295271 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37673
GenBank
L14758
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence