LOCUS       sequence-100003-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with C-term sstFRET inserted to measure 
            cytoskeletal stress in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Meng F, Sachs F
  TITLE     Visualizing dynamic cytoplasmic forces with a compliance-matched 
            FRET sensor.
  JOURNAL   J Cell Sci. 2011 Jan 15;124(Pt 2):261-9. doi: 10.1242/jcs.071928. 
            Epub 2010 Dec 20.
  PUBMED    21172803
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2011-01-15"; volume: "124(Pt 2):261-9. doi"; pages: " 
            10.1242/jcs.071928. Epub 2010 Dec 20"
COMMENT     Sequence Label: PEG-Actinin-C-sstFRET
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(175..426)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(217..238)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     566..585
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc
      121 ggtggatccc gggcccgcgg taccttagcg gccgcttgga tcccgggccc tcttcttgta
      181 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      241 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
      301 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      361 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      421 cttgtcggcg gtgatataga cgttgtcgct gatggcgttg tactccagct tgtgccccag
      481 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      541 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      601 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      661 gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
      721 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      781 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgt
//
