LOCUS       sequence-100004-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with C-term sstFRET inserted to measure 
            cytoskeletal stress in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Meng F, Sachs F
  TITLE     Visualizing dynamic cytoplasmic forces with a compliance-matched 
            FRET sensor.
  JOURNAL   J Cell Sci. 2011 Jan 15;124(Pt 2):261-9. doi: 10.1242/jcs.071928. 
            Epub 2010 Dec 20.
  PUBMED    21172803
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2011-01-15"; volume: "124(Pt 2):261-9. doi"; pages: " 
            10.1242/jcs.071928. Epub 2010 Dec 20"
COMMENT     Sequence Label: PEG-Actinin-C-sstFRET
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(493..744)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(535..556)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatggaat tcacgcctcc
      181 ctttgccttg cgctgatctg cacgtttccc caggctgctc caggcctggt ttaactctgt
      241 gcacttttcc tgcaggtctt ctgctgactc gggatgggac tggatcaggc gctctgctgt
      301 ttcaccaagg gagtttacct tgtcaccgag agccgcaagg tccctctcga agccctcatg
      361 cttgcgttgc agggcctgga cactggcgag atcatgtcca taattgtctg tgtttagagc
      421 ttgattcttc tcttcaatcc attctttggt ttcatcagca tctctgtgga agcctccaga
      481 tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      541 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagtt tggactggta
      601 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      661 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
      721 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctg
//
