LOCUS       sequence-100005-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with C-term sstFRET inserted to measure 
            cytoskeletal stress in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Meng F, Sachs F
  TITLE     Visualizing dynamic cytoplasmic forces with a compliance-matched 
            FRET sensor.
  JOURNAL   J Cell Sci. 2011 Jan 15;124(Pt 2):261-9. doi: 10.1242/jcs.071928. 
            Epub 2010 Dec 20.
  PUBMED    21172803
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2011-01-15"; volume: "124(Pt 2):261-9. doi"; pages: " 
            10.1242/jcs.071928. Epub 2010 Dec 20"
COMMENT     Sequence Label: PEG-Actinin-C-sstFRET
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg caccggtgag
      181 gtcactctcg ccgtacagcg ccgtggagaa ggacatgtag tccagagcac ctggcacgga
      241 gtcggggccg gtgtaggggg ccatccgcgc gatgcagtac tcagcctggt cgggtggcag
      301 ctcgcggcgc agctcgtcca tggtaatgta gttcttgtcc ccagccagga tcttgaagga
      361 agccatgact tggtctgctg tatctgtgtc ggctgtctcg cgggacatga agtcaatgaa
      421 ggcctggaat gtcactaccc ccaggcggtt ggggtccaca atgctcatga tgcgggcaaa
      481 ttctgcttct ccctgggggt cgttgccaat atcataaccc aagctgatga ggcaggcttt
      541 gaactcctcg ggacccagtg tgccggagtg atcccggtca aagtggttga aggaggcccg
      601 gaactcattc atctgctcct ggctgatgcc cttggcatcc cgggtcagga tctggttctc
      661 tacctcattg atggtcctgg cgatggtggt gagcagctgc tcccagccca cacggatgtg
      721 ctccatggtg tagttggtgt gcttgttgtc gaagatgagc gcctcctgga tgagctggtg
      781 gtcgccctcc agctgatcaa tctttggctt gtagttgacg atgctcttct c
//
