LOCUS       sequence-100010-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Filamin with internal stFRET inserted to measure 
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Filamin-M-stFRET
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(289..540)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(331..352)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     680..699
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc accatggaat tcacacctgc
      181 aagtatcggc acgaaaccct ggattttctg accaattttt ttaagctttt ctttaatctt
      241 gtttctaaat tttcgtaagc gcttgcgcag cccagatctg agtccggact tgtacagctc
      301 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      361 cttctcgttg gggtctttgc tcagtttgga ctggtagctc aggtagtggt tgtcgggcag
      421 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc
      481 gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc
      541 ggcggtgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      601 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      661 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      721 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      781 gtagcgggcg aagcactgca ggccgtagcc cagggtggtc acga
//
