LOCUS       sequence-100014-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with internal stFRET inserted to measure
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Actinin-M-stFRET
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..120
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggcagcttg ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc
       61 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
      121 gatgggcacc accccggtga acagctcctc gcccttgctc acggatccca gctgaagctt
      181 tccgccaccg gtccggttct ccagccacgg gattgtgcgg cggatccact ccaacagatc
      241 actggccagc ttctcgtagt cttccataag ctgctcgttc tcctggttga cggccaacac
      301 cttgcagatg cgattggctg ctgtctccgc cttctgggct ccagagaagg cgtggtagaa
      361 gctagacacg taagtcatga tggctttctc atccggtcgg gcagttccaa cgatgtcttc
      421 ggcatccagc atcttgggga tgtccaggta cttctctgcc acgtcaaaag ccgtattcag
      481 atttgtgagt ggatcatcct tccgcagctt cccgtagtca atcagctcgg gccggtgtcg
      541 gtggatcaaa gcacagaagc cgaggccatc cttccagctt atgtggaagt tctggatgtt
      601 gacatttttg taaggggctg tctttctctg acaccacagg agcagccctt ccttggctga
      661 agtctcttcc acggagatgt cctggatggc aaagcgcagg atgatggtcc agatcatgcc
      721 cagggtcatc ttcacattcc catccacgat ttcttcggct ccgatggaca ccagtttgac
      781 gcctttgctg gctatgaaat ccagggcctt gttgacgttg gagatcttgt gcactctcat
      841 cttgcctcgc tctggcttgg ccaagcgtt
//
