LOCUS       sequence-100019-        4024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible SCRIBE_galK(OFF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF714
FEATURES             Location/Qualifiers
     source          1..4024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..33
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1320..2105
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     terminator      2123..2209
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2373..2961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2453..2472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3049..3143)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3164..3823)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3738..3757
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3824..3926)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3992..4010
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct gaattcggaa actgctttgg tccccagtga gcggcaatcg attcattagg
      241 catgatcttt cttgccgagc gcggaaatta gctggaattc aggaaacccg ttttttctga
      301 cgtaagggtg cgcaactttc atgaaatccg ctgaatattt gaacactttt agattgagaa
      361 atctcggcct acctgtcatg aacaatttgc atgacatgtc taaggcgact cgcatatctg
      421 ttgaaacact tcggttgtta atctatacag ctgattttcg ctataggatc tacactgtag
      481 aaaagaaagg cccagagaag agaatgagaa ccatttacca accttctcga gaacttaaag
      541 ccttacaagg atgggttcta cgtaacattt tagataaact gtcgtcatct cctttttcta
      601 ttggatttga aaagcaccaa tctattttga ataatgctac cccgcatatt ggggcaaact
      661 ttatactgaa tattgatttg gaggattttt tcccaagttt aactgctaac aaagtttttg
      721 gagtgttcca ttctcttggt tataatcgac taatatcttc agttttgaca aaaatatgtt
      781 gttataaaaa tctgctacca caaggtgctc catcatcacc taaattagct aatctaatat
      841 gttctaaact tgattatcgt attcagggtt atgcaggtag tcggggcttg atatatacga
      901 gatatgccga tgacctcacc ttatctgcac agtctatgaa aaaggttgtt aaagcacgtg
      961 attttttatt ttctataatc ccaagtgaag gattggttat taactcaaaa aaaacttgta
     1021 ttagtgggcc tcgtagtcag aggaaagtta caggtttagt tatttcacaa gagaaagttg
     1081 ggataggtag agaaaaatat aaagaaatta gagcaaagat acatcatata ttttgcggta
     1141 agtcttctga gatagaacac gttaggggat ggttgtcatt tattttaagt gtggattcaa
     1201 aaagccatag gagattaata acttatatta gcaaattaga aaaaaaatat ggaaagaacc
     1261 ctttaaataa agcgaagacc taaggatccg gttgatattg attcagaggt ataaaacgaa
     1321 tgagtactgc actcgcaacg ctggctggga agctggctga acgtgtcggc atggattctg
     1381 tcgacccaca ggaactgatc accactcttc gccagacggc atttaaaggt gatgccagcg
     1441 atgcgcagtt catcgcatta ctgatcgttg ccaaccagta cggccttaat ccgtggacga
     1501 aagaaattta cgcctttcct gataagcaga atggcatcgt tccggtggtg ggcgttgatg
     1561 gctggtcccg catcatcaat gaaaaccagc agtttgatgg catggacttt gagcaggaca
     1621 atgaatcctg tacatgccgg atttaccgca aggaccgtaa tcatccgatc tgcgttaccg
     1681 aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg cgaaggcaga gaaatcacgg
     1741 ggccgtggca gtcgcatccc aaacggatgt tacgtcataa agccatgatt cagtgtgccc
     1801 gtctggcctt cggatttgct ggtatctatg acaaggatga agccgagcgc attgtcgaaa
     1861 atactgcata cactgcagaa cgtcagccgg aacgcgacat cactccggtt aacgatgaaa
     1921 ccatgcagga gattaacact ctgctgatcg ccctggataa aacatgggat gacgacttat
     1981 tgccgctctg ttcccagata tttcgccgcg acattcgtgc atcgtcagaa ctgacacagg
     2041 ccgaagcagt aaaagctctt ggattcctga aacagaaagc cgcagagcag aaggtggcag
     2101 catgaacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2161 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
     2221 gacctaggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat
     3061 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     3121 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     3181 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga
     3241 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     3301 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     3361 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     3421 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     3481 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     3541 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     3601 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     3661 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     3721 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     3781 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     3841 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     3901 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg acgtctaaga
     3961 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4021 tcac
//
