LOCUS       sequence-100020-        4021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible SCRIBE_kanR(ON).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF745
FEATURES             Location/Qualifiers
     source          1..4021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..33
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1317..2102
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     terminator      2120..2206
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2370..2958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2450..2469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3046..3140)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3161..3820)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3735..3754
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3821..3923)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3989..4007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct gaattccgat agattgtcgc acctgattgc ccgacattat cgcgggccca
      241 tttataccca tataaatcag catccatgtt ggaattcagg aaacccgttt tttctgacgt
      301 aagggtgcgc aactttcatg aaatccgctg aatatttgaa cacttttaga ttgagaaatc
      361 tcggcctacc tgtcatgaac aatttgcatg acatgtctaa ggcgactcgc atatctgttg
      421 aaacacttcg gttgttaatc tatacagctg attttcgcta taggatctac actgtagaaa
      481 agaaaggccc agagaagaga atgagaacca tttaccaacc ttctcgagaa cttaaagcct
      541 tacaaggatg ggttctacgt aacattttag ataaactgtc gtcatctcct ttttctattg
      601 gatttgaaaa gcaccaatct attttgaata atgctacccc gcatattggg gcaaacttta
      661 tactgaatat tgatttggag gattttttcc caagtttaac tgctaacaaa gtttttggag
      721 tgttccattc tcttggttat aatcgactaa tatcttcagt tttgacaaaa atatgttgtt
      781 ataaaaatct gctaccacaa ggtgctccat catcacctaa attagctaat ctaatatgtt
      841 ctaaacttga ttatcgtatt cagggttatg caggtagtcg gggcttgata tatacgagat
      901 atgccgatga cctcacctta tctgcacagt ctatgaaaaa ggttgttaaa gcacgtgatt
      961 ttttattttc tataatccca agtgaaggat tggttattaa ctcaaaaaaa acttgtatta
     1021 gtgggcctcg tagtcagagg aaagttacag gtttagttat ttcacaagag aaagttggga
     1081 taggtagaga aaaatataaa gaaattagag caaagataca tcatatattt tgcggtaagt
     1141 cttctgagat agaacacgtt aggggatggt tgtcatttat tttaagtgtg gattcaaaaa
     1201 gccataggag attaataact tatattagca aattagaaaa aaaatatgga aagaaccctt
     1261 taaataaagc gaagacctaa ggatccggtt gatattgatt cagaggtata aaacgaatga
     1321 gtactgcact cgcaacgctg gctgggaagc tggctgaacg tgtcggcatg gattctgtcg
     1381 acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg
     1441 cgcagttcat cgcattactg atcgttgcca accagtacgg ccttaatccg tggacgaaag
     1501 aaatttacgc ctttcctgat aagcagaatg gcatcgttcc ggtggtgggc gttgatggct
     1561 ggtcccgcat catcaatgaa aaccagcagt ttgatggcat ggactttgag caggacaatg
     1621 aatcctgtac atgccggatt taccgcaagg accgtaatca tccgatctgc gttaccgaat
     1681 ggatggatga atgccgccgc gaaccattca aaactcgcga aggcagagaa atcacggggc
     1741 cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag tgtgcccgtc
     1801 tggccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt gtcgaaaata
     1861 ctgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac gatgaaacca
     1921 tgcaggagat taacactctg ctgatcgccc tggataaaac atgggatgac gacttattgc
     1981 cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg acacaggccg
     2041 aagcagtaaa agctcttgga ttcctgaaac agaaagccgc agagcagaag gtggcagcat
     2101 gaacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2161 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
     2221 ctaggcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3001 aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa
     3061 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     3121 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     3181 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
     3241 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     3301 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     3361 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     3421 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     3481 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     3541 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     3601 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     3661 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     3721 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     3781 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     3841 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     3901 tggaacctct tacgtgccga tcaacgtctc attttcgcca gatatcgacg tctaagaaac
     3961 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     4021 c
//
