LOCUS       sequence-100022-        3211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible msd_kanR(ON)_dRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF749
FEATURES             Location/Qualifiers
     source          1..3211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..33
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1310..1396
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1560..2148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1640..1659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2236..2330)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2351..3010)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2925..2944
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3011..3113)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3179..3197
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct gaattccgat agattgtcgc acctgattgc ccgacattat cgcgggccca
      241 tttataccca tataaatcag catccatgtt ggaattcagg aaacccgttt tttctgacgt
      301 aagggtgcgc aactttcatg aaatccgctg aatatttgaa cacttttaga ttgagaaatc
      361 tcggcctacc tgtcatgaac aatttgcatg acatgtctaa ggcgactcgc atatctgttg
      421 aaacacttcg gttgttaatc tatacagctg attttcgcta taggatctac actgtagaaa
      481 agaaaggccc agagaagaga atgagaacca tttaccaacc ttctcgagaa cttaaagcct
      541 tacaaggatg ggttctacgt aacattttag ataaactgtc gtcatctcct ttttctattg
      601 gatttgaaaa gcaccaatct attttgaata atgctacccc gcatattggg gcaaacttta
      661 tactgaatat tgatttggag gattttttcc caagtttaac tgctaacaaa gtttttggag
      721 tgttccattc tcttggttat aatcgactaa tatcttcagt tttgacaaaa atatgttgtt
      781 ataaaaatct gctaccacaa ggtgctccat catcacctaa attagctaat ctaatatgtt
      841 ctaaacttga ttatcgtatt cagggttatg caggtagtcg gggcttgata tatacgagat
      901 atgccgctgc tctcacctta tctgcacagt ctatgaaaaa ggttgttaaa gcacgtgatt
      961 ttttattttc tataatccca agtgaaggat tggttattaa ctcaaaaaaa acttgtatta
     1021 gtgggcctcg tagtcagagg aaagttacag gtttagttat ttcacaagag aaagttggga
     1081 taggtagaga aaaatataaa gaaattagag caaagataca tcatatattt tgcggtaagt
     1141 cttctgagat agaacacgtt aggggatggt tgtcatttat tttaagtgtg gattcaaaaa
     1201 gccataggag attaataact tatattagca aattagaaaa aaaatatgga aagaaccctt
     1261 taaataaagc gaagacctaa ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg
     1321 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1381 cctgagtagg acaaatccgc cgccctagac ctaggcgttc ggctgcggcg agcggtatca
     1441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1741 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2221 atgactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     2281 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     2341 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     2401 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     2461 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     2521 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     2581 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     2641 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2701 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2761 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     2821 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2881 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2941 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3001 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     3061 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     3121 attttcgcca gatatcgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3181 taggcgtatc acgaggccct ttcgtcttca c
//
