LOCUS       sequence-100024-        3166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible msd(wt)_dRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF758
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..33
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    40..56
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1265..1351
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1515..2103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1595..1614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2191..2285)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2306..2965)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2880..2899
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2966..3068)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3134..3152
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagatgc tagcaattgt gagcggataa caattgacat tgtgagcgga taacaagata
       61 ctgagcacat cagcaggacg cactgacctt aattaaatgc gcacccttag cgagaggttt
      121 atcattaagg tcaacctctg gatgttgttt cggcatcctg cattgaatct gagttactgt
      181 ctgttttcct tgttggaacg gagagcatcg cctgatgctc tccgagccaa ccaggaaacc
      241 cgttttttct gacgtaaggg tgcgcaactt tcatgaaatc cgctgaatat ttgaacactt
      301 ttagattgag aaatctcggc ctacctgtca tgaacaattt gcatgacatg tctaaggcga
      361 ctcgcatatc tgttgaaaca cttcggttgt taatctatac agctgatttt cgctatagga
      421 tctacactgt agaaaagaaa ggcccagaga agagaatgag aaccatttac caaccttctc
      481 gagaacttaa agccttacaa ggatgggttc tacgtaacat tttagataaa ctgtcgtcat
      541 ctcctttttc tattggattt gaaaagcacc aatctatttt gaataatgct accccgcata
      601 ttggggcaaa ctttatactg aatattgatt tggaggattt tttcccaagt ttaactgcta
      661 acaaagtttt tggagtgttc cattctcttg gttataatcg actaatatct tcagttttga
      721 caaaaatatg ttgttataaa aatctgctac cacaaggtgc tccatcatca cctaaattag
      781 ctaatctaat atgttctaaa cttgattatc gtattcaggg ttatgcaggt agtcggggct
      841 tgatatatac gagatatgcc gctgctctca ccttatctgc acagtctatg aaaaaggttg
      901 ttaaagcacg tgatttttta ttttctataa tcccaagtga aggattggtt attaactcaa
      961 aaaaaacttg tattagtggg cctcgtagtc agaggaaagt tacaggttta gttatttcac
     1021 aagagaaagt tgggataggt agagaaaaat ataaagaaat tagagcaaag atacatcata
     1081 tattttgcgg taagtcttct gagatagaac acgttagggg atggttgtca tttattttaa
     1141 gtgtggattc aaaaagccat aggagattaa taacttatat tagcaaatta gaaaaaaaat
     1201 atggaaagaa ccctttaaat aaagcgaaga cctaaggatc ccatggtacg cgtgctagag
     1261 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1321 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg cgttcggctg
     1381 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1441 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1501 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1561 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1621 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1681 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     1741 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1801 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1861 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1921 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     1981 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2041 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2101 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2161 taagggattt tggtcatgac tagtgcttgg attctcacca ataaaaaacg cccggcggca
     2221 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     2281 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     2341 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     2401 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     2461 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     2521 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     2581 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     2641 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     2701 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     2761 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     2821 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2881 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2941 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     3001 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     3061 gccgatcaac gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac
     3121 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcac
//
