LOCUS       sequence-100026-        4092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AHL-inducible msd_lacZ(ON).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF828
FEATURES             Location/Qualifiers
     source          1..4092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..33)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        99..201
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             202..861
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(268..287)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      882..976
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1064..1652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1553..1572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1816..1902)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
       61 ggtttcttag acgtcgatat ctggcgaaaa tgagacgttg atcggcacgt aagaggttcc
      121 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      181 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
      241 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
      301 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
      361 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
      421 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
      481 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      541 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      601 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      661 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      721 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      781 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      841 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
      901 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      961 ttttattggt gagaatccaa gcactagtca tgaccaaaat cccttaacgt gagttttcgt
     1021 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1081 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1141 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1201 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1261 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1321 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1381 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1441 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1501 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1561 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1621 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1681 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1741 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgccta
     1801 ggtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     1861 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctct agcacgcgta
     1921 ccatgggatc cttaggtctt cgctttattt aaagggttct ttccatattt tttttctaat
     1981 ttgctaatat aagttattaa tctcctatgg ctttttgaat ccacacttaa aataaatgac
     2041 aaccatcccc taacgtgttc tatctcagaa gacttaccgc aaaatatatg atgtatcttt
     2101 gctctaattt ctttatattt ttctctacct atcccaactt tctcttgtga aataactaaa
     2161 cctgtaactt tcctctgact acgaggccca ctaatacaag ttttttttga gttaataacc
     2221 aatccttcac ttgggattat agaaaataaa aaatcacgtg ctttaacaac ctttttcata
     2281 gactgtgcag ataaggtgag gtcatcggca tatctcgtat atatcaagcc ccgactacct
     2341 gcataaccct gaatacgata atcaagttta gaacatatta gattagctaa tttaggtgat
     2401 gatggagcac cttgtggtag cagattttta taacaacata tttttgtcaa aactgaagat
     2461 attagtcgat tataaccaag agaatggaac actccaaaaa ctttgttagc agttaaactt
     2521 gggaaaaaat cctccaaatc aatattcagt ataaagtttg ccccaatatg cggggtagca
     2581 ttattcaaaa tagattggtg cttttcaaat ccaatagaaa aaggagatga cgacagttta
     2641 tctaaaatgt tacgtagaac ccatccttgt aaggctttaa gttctcgaga aggttggtaa
     2701 atggttctca ttctcttctc tgggcctttc ttttctacag tgtagatcct atagcgaaaa
     2761 tcagctgtat agattaacaa ccgaagtgtt tcaacagata tgcgagtcgc cttagacatg
     2821 tcatgcaaat tgttcatgac aggtaggccg agatttctca atctaaaagt gttcaaatat
     2881 tcagcggatt tcatgaaagt tgcgcaccct tacgtcagaa aaaacgggtt tcctgaattc
     2941 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3001 gcccgcaccg atcgccctga attcaggaaa acagacagta actcagattc aatgcaggat
     3061 gccgaaacaa catccagagg ttgaccttaa tgataaacct ctcgctaagg gtgcgcattt
     3121 aattaatatt cgactataac aaaccatttt cttgcgtaaa cctgtacgat cctacaggtg
     3181 cttatgttaa gtaattgtat tcccagcgat acaatagtgt gacaaaaatc caatttatta
     3241 gaatcaaatg tcaatccatt accgttttaa tgatatataa cacgcaaaac ttgcgacaaa
     3301 caataggtaa ggataaagag atgggtatga aaaacataaa tgccgacgac acatacagaa
     3361 taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc ttatctgata
     3421 tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct cattctatgg
     3481 ttaaatctga tatttcaatt ctagataatt accctaaaaa atggaggcaa tattatgatg
     3541 acgctaattt aataaaatat gatcctatag tagattattc taactccaat cattcaccaa
     3601 ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat gtaattaaag
     3661 aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg gctaacaatg
     3721 gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat agtttatttt
     3781 tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat tatcgaaaaa
     3841 taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa gaatgtttag
     3901 cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggc tgcagtgagc
     3961 gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca aaccgctgcc
     4021 aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt aaaaattaac
     4081 tagcatctcg ag
//
