LOCUS       sequence-100044-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Filamin with C-term stFRET inserted to measure 
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Filamin-C-stFRET
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(187..210)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(211..462)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(253..274)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     602..621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
       61 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat ccggtggatc
      121 ccgggcccgc ggtaccgtcg actgcagaat tcgaagcttg agctcttagc ggccgctgcc
      181 acctccgtgg tgatgatggt ggtgatgatg cttgtacagc tcgtccatgc cgagagtgat
      241 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      301 gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      361 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      421 gcggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt
      481 gtcgctgatg gcgttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      541 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      601 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      661 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg
      721 cacgccccag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      781 gcagatgaac ttcagggtca gcttgccgta
//
