LOCUS       sequence-100055-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Spectrin-C-term cpstFRET to measure mechanical stress 
            and report multidimensional stress fields.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Meng F, Sachs F
  TITLE     Orientation-based FRET sensor for real-time imaging of cellular 
            forces.
  JOURNAL   J Cell Sci. 2012 Feb 1;125(Pt 3):743-50. doi: 10.1242/jcs.093104.
  PUBMED    22389408
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-02-1"; volume: "125(Pt 3):743-50. doi"; pages: " 
            10.1242/jcs.093104"
COMMENT     Sequence Label: Spectrin-C-cpstFRET
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(144..161)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(join(174..686,687..689))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     367..386
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     626..647
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtgcggc cgctgccacc ggaactaccg cgtggcacca gagcgagctc gtcctcgatg
      181 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      241 acgttgtcgc tgatggcgtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      301 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      361 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      421 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      481 cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      541 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      601 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      661 ccggtgaaca gctcctcgcc cttgctcacc ccgccaccgc cgccgaattc cttgtacagc
      721 tcgtccatgc cgagagtgat cccggcggcg gtcacg
//
