LOCUS       sequence-100065-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha-actinin with C-term stFRET inserted to measure 
            mechanical stress in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Meng F, Suchyna TM, Sachs F
  TITLE     A fluorescence energy transfer-based mechanical stress sensor for 
            specific proteins in situ.
  JOURNAL   FEBS J. 2008 Jun;275(12):3072-87. doi: 
            10.1111/j.1742-4658.2008.06461.x. Epub 2008 May 10.
  PUBMED    18479457
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2008.06461.x"; journalName: "FEBS J"; date: 
            "2008-06"; volume: "275"; issue: "12"; pages: "3072-87"
COMMENT     Sequence Label: Actinin-C-stFRET
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     100..121
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgggcagctt gccggtggtg cagatcagct tcagggtcag cttgccgtag gtggcatcgc
       61 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
      121 ggatgggcac caccccggtg aacagctcct cgcccttgct cacggatccc agctgaagct
      181 ttccgccacc ggtgaggtca ctctcgccgt acagcgccgt ggagaaggac atgtagtcca
      241 gagcacctgg cacggagtcg gggccggtgt agggggccat ccgcgcgatg cagtactcag
      301 cctggtcggg tggcagctcg cggcgcagct cgtccatggt aatgtagttc ttgtccccag
      361 ccaggatctt gaaggaagcc atgacttggt ctgctgtatc tgtgtcggct gtctcgcggg
      421 acatgaagtc aatgaaggcc tggaatgtca ctacccccag gcggttgggg tccacaatgc
      481 tcatgatgcg ggcaaattct gcttctccct gggggtcgtt gccaatatca taacccaagc
      541 tgatgaggca ggctttgaac tcctcgggac ccagtgtgcc ggagtgatcc cggtcaaagt
      601 ggttgaagga ggcccggaac tcattcatct gctcctggct gatgcccttg gcatcccggg
      661 tcaggatctg gttctctacc tcattgatgg tcctggcgat ggtggtgagc agctgctccc
      721 agcccacacg gatgtgctcc atggtgtagt tggtgtgctt gttgtcgaag atgagcgcct
      781 cctggatgag ctggtggtcg ccctccagct gatcaatctt tggcttgtag ttgacgatgc
      841 tcttctcata ctgccgca
//
