LOCUS       sequence-100070-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Spectrin-cpstFRET to measure mechanical stress and 
            report multidimensional stress fields .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Meng F, Sachs F
  TITLE     Orientation-based FRET sensor for real-time imaging of cellular 
            forces.
  JOURNAL   J Cell Sci. 2012 Feb 1;125(Pt 3):743-50. doi: 10.1242/jcs.093104.
  PUBMED    22389408
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-02-1"; volume: "125(Pt 3):743-50. doi"; pages: " 
            10.1242/jcs.093104"
COMMENT     Sequence Label: Spectrin-M-cpstFRET
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(84..86,87..599)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(126..147)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(387..406)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
       61 aggaattcgg cggcggtggc ggggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 ac
//
