LOCUS       sequence-100075-       13122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector (OsUBI promoter) for direct cloning of 
            artificial microRNAs into rice MIR390 precursor. To use for 
            expressing amiRNAs in monocots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Carbonell A, Fahlgren N, Mitchell S, Cox KL Jr, Mockler TC, 
            Carrington JC
  TITLE     Highly Specific Gene Silencing in a Monocot Species by Artificial 
            MicroRNAs Derived From Chimeric MIRNA Precursors.
  JOURNAL   Plant J. 2015 Mar 24. doi: 10.1111/tpj.12835.
  PUBMED    25809382
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2015 Mar 24. doi: 10.1111/tpj.12835."
COMMENT     Sequence Label: pH7WG2B-OsMIR390-B/c
FEATURES             Location/Qualifiers
     source          1..13122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        202..1907
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          950..1907
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    1942..1966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(2051..2056,2057..2074,2075..2081)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2135..2794
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2201..2220)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3136..3441
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3320..3339
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3545..3569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5231..6256
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     primer_bind     complement(6271..6287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6271..6287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6284..6306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6295..6311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6319..6325,6326..6343,6344..6349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6364..6385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6702..6726
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7254..8045
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8289..8877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8778..8797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9031..9048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9063..9203
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9129..9148)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9289..9311
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9547..9741
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9807..10874)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(11308..11937)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
       61 cttttcacgc ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctaagct tattcgggtc aaggcggaag ccagcgcgcc accccacgtc
      241 agcaaatacg gaggcgcggg gttgacggcg tcacccggtc ctaacggcga ccaacaaacc
      301 agccagaaga aattacagta aaaaaaaagt aaattgcact ttgatccacc ttttattacc
      361 taagtctcaa tttggatcac ccttaaacct atcttttcaa tttgggccgg gttgtggttt
      421 ggactaccat gaacaacttt tcgtcatgtc taacttccct ttcagcaaac atatgaacca
      481 tatatagagg agatcggccg tatactagag ctgatgtgtt taaggtcgtt gattgcacga
      541 gaaaaaaaaa tccaaatcgc aacaatagca aatttatctg gttcaaagtg aaaagatatg
      601 tttaaaggta gtccaaagta aaacttatag ataataaaat gtggtccaaa gcgtaattca
      661 ctcaaaaaaa atcaacgaga cgtgtaccaa acggagacaa acggcatctt ctcgaaattt
      721 cccaaccgct cgctcgcccg cctcgtcttc ccggaaaccg cggtggtttc agcgtggcgg
      781 attctccaag cagacggaga cgtcacggca cgggactcct cccaccaccc aaccgccata
      841 aataccagcc ccctcatctc ctctcctcgc atcagctcca cccccgaaaa atttctcccc
      901 aatctcgcga ggctctcgtc gtcgaatcga atcctctcgc gtcctcaagg tacgctgctt
      961 ctcctctcct cgcttcgttt cgattcgatt tcggacgggt gaggttgttt tgttgctaga
     1021 tccgattggt ggttagggtt gtcgatgtga ttatcgtgag atgtttaggg gttgtagatc
     1081 tgatggttgt gatttgggca cggttggttc gataggtgga atcgtggtta ggttttggga
     1141 ttggatgttg gttctgatga ttggggggaa tttttacggt tagatgaatt gttggatgat
     1201 tcgattgggg aaatcggtgt agatctgttg gggaattgtg gaactagtca tgcctgagtg
     1261 attggtgcga tttgtagcgt gttccatctt gtaggccttg ttgcgagcat gttcagatct
     1321 actgttccgc tcttgattga gttattggtg cggttggtgc aaacacaggc tttaatatgt
     1381 tatatctgtt ttgtgtttga tgtagatctg tagggtagtt cttcttagac atggttcaat
     1441 tatgtagctt gtgcgtttcg atttgatttc atatgttcac agattagata atgatgaact
     1501 cttttaatta attgtcaatg gtaaatagga agtcttgtcg ctatatctgt cataatgatc
     1561 tcatgttact atctgccagt aatttatgct aagaactata ttagaatatc atgttacaat
     1621 ctgtagtaat atcatgttac aatctgtagt tcatctatat aatctattgt ggtaatttct
     1681 ttttactatc tgtgtgaaga ttattgccac tagttcattc tacttatttc tgaagttcag
     1741 gatacgtgtg ctgttactac ctatctgaat acatgtgtga tgtgcctgtt actatctttt
     1801 tgaatacatg tatgttctgt tggaatatgt ttgctgtttg atccgttgtt gtgtccttaa
     1861 tcttgtgcta gttcttaccc tatctgtttg gtgattattt cttgcagatt cagatcgggc
     1921 ccaagcttga ctagtgatat cacaagtttg tacaaaaaag caggctccgc ggccgccccc
     1981 ttcaccgagc tcgagatgtt ttgaggaagg gtatggaaca atccttgaga gaccattagg
     2041 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     2101 gccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     2161 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2221 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2281 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2341 tcatccggag ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2401 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2461 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2521 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2581 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2641 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2701 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2761 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggagccggct tactaaaagc
     2821 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2881 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2941 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     3001 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     3061 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     3121 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     3181 ctgtttgtgg atgtacagag tgatattatt gacacgcccg gccgacggat ggtgatcccc
     3241 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3301 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtttccgtt
     3361 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3421 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcacctcga
     3481 cggtctcaca tggtttgttc ttaccacacg accaattaaa tcgagctcaa gggtgggcgc
     3541 gccgacccag ctttcttgta caaagtggtg atatcccgcg gccatgctag agtccgcaaa
     3601 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga
     3661 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     3721 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     3781 cctaaaacca aaatccagtg acctgcaggc atgcgacgtc gggccctcta gaggatcccc
     3841 gggtaccgtg cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct
     3901 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc
     3961 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct
     4021 ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct
     4081 cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc
     4141 ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca
     4201 aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc ggcgttccgg
     4261 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt
     4321 gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg
     4381 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca
     4441 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc
     4501 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg
     4561 tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaaatctgt
     4621 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca
     4681 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat
     4741 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg
     4801 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct
     4861 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt
     4921 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
     4981 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
     5041 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
     5101 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
     5161 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtcgactc
     5221 tagaggatcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
     5281 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
     5341 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
     5401 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
     5461 gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
     5521 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga
     5581 ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
     5641 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
     5701 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
     5761 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
     5821 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
     5881 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
     5941 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
     6001 aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
     6061 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
     6121 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     6181 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     6241 gagggcaaag aaataggaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     6301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6361 ctaatgagtg agctaactca cattacttaa gattgaatcc tgttgccggt cttgcgatga
     6421 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     6481 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     6541 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     6601 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa
     6661 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     6721 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     6781 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     6841 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     6901 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     6961 atcaattcgg gcacgaaccc agtggacata agcctcgttc ggttcgtaag ctgtaatgca
     7021 agtagcgtaa ctgccgtcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     7081 cggcgcagtg gcggttttca tggcttcttg ttatgacatg tttttttggg gtacagtcta
     7141 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     7201 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     7261 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     7321 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     7381 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     7441 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     7501 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     7561 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     7621 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     7681 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     7741 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     7801 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     7861 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     7921 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     7981 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     8041 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgccggcgt taactcaagc
     8101 gattagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     8161 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     8221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8401 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9061 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     9121 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     9181 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     9241 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9301 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     9361 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     9421 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     9481 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     9541 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     9601 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     9661 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     9721 ccaatgtacg tgctatccac aggaaagaga acttttcgac ctttttcccc tgctagggca
     9781 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     9841 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     9901 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     9961 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    10021 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    10081 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    10141 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    10201 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    10261 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    10321 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    10381 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    10441 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    10501 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    10561 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    10621 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    10681 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    10741 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    10801 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    10861 gatggtttgc gaccgtcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg
    10921 ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca
    10981 ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa
    11041 aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag
    11101 atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat
    11161 cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca
    11221 acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt
    11281 tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag
    11341 cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc
    11401 ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc
    11461 gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga
    11521 cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc
    11581 caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa
    11641 gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac
    11701 tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc
    11761 gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg
    11821 atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt
    11881 tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg
    11941 ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc
    12001 aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg
    12061 gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc
    12121 agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc
    12181 gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg
    12241 cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt
    12301 cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa
    12361 gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc
    12421 ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc
    12481 gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga
    12541 tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg
    12601 ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct
    12661 ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg
    12721 tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt
    12781 cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt
    12841 cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc
    12901 cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact
    12961 ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc
    13021 gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga
    13081 agccgactgc actatagcag cggaggggtt ggatcaaagt ac
//
