LOCUS       sequence-100077-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-H2B-6
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(41..418)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(507..710)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(536..556)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc cttagcgctg gtgtacttgg
       61 tgatggcctt agtaccctcg gacacggcgt gcttggccaa ctccccaggc agcagcaggc
      121 gcacggccgt ctggatctcc ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc
      181 gggaagcctc acctgcgatg cgctcgaaaa tgtcgttcac aaacgaattc atgatgccca
      241 tggccttgga cgaaatgccg gtgtcagggt ggacctgctt cagaaccttg tacacataga
      301 tggaatagct ctccttgcgg ctgcgcttgc gcttcttgcc gcctttcttc tgcgccttag
      361 tcaccgcctt cttggagccc tttttcgggg cgggagcaga cttcgctggc tctggcatgg
      421 tggcggtacc gtcgactgca gaattcgaag cttgagctcg agatctgagt ccggtagcgc
      481 tagcggatct gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc
      541 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
      601 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
      661 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
      721 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
      781 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa taggg
//
