LOCUS       sequence-100098-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  eGFP-expressing lentiviral vector with IRES and pA-signal; suitable 
            for use as NRTLV (non-reverse transcribable lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Mock U, Riecken K, Berdien B, Qasim W, Chan E, Cathomen T, Fehse B
  TITLE     Novel lentiviral vectors with mutated reverse transcriptase for mRNA
            delivery of TALE nucleases.
  JOURNAL   Sci Rep. 2014 Sep 18;4:6409. doi: 10.1038/srep06409.
  PUBMED    25230987
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-09-18"; volume: "4:6409. doi"; pages: " 
            10.1038/srep06409"
COMMENT     Sequence Label: LeGO-iG2-wPRE-pA
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..113
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
       61 acagcagaga tccagtttgg ttagtaccgg gcccgccgag gtcgacggta tcgataagct
      121 cgcttcacga gattccagca ggtcgaggga cctaataact tcgtatagca tacattatac
      181 gaagttatat taagggttcc aagcttaagc ggccggccgc tgaaagaccc cacctgtagg
      241 tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga
      301 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga
      361 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
      421 tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt
      481 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
      541 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
      601 ctctataa
//
