LOCUS       sequence-100100-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression vector for Cre-recombinase containing MET15 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   MacDonald C, Piper RC
  TITLE     Puromycin and Methotrexate Resistance Cassettes and Optimized 
            cre-recombinase Expression Plasmids for use in Yeast.
  JOURNAL   Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.
  PUBMED    25688547
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Feb 16. doi: 10.1002/yea.3069."
COMMENT     Sequence Label: pPL5628_TEF1*-Cre_MET15
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..44)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    85..588
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        625..729
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(948..967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
       61 atgataataa tggtttctta gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct
      121 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
      181 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa
      241 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga
      301 aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat
      361 tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc
      421 aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt
      481 cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata
      541 tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca
      601 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      661 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      721 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      781 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      841 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      901 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      961 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1021 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1081 agagaattat gcagtgctgc cataaccatg agtgataaca
//
