LOCUS       sequence-100115-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses calpain1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Wang Y, Zhang Y
  TITLE     Regulation of TET protein stability by calpains.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):278-84. doi: 
            10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.
  PUBMED    24412366
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.031"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "278-84"
COMMENT     Sequence Label: p3XFlag-CAPN1
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(194..259)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatca ctacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
      241 cgtcatggtc tttgtagtca gcccgggatc ctgcaaacat ggtcagctgc aaccacttaa
      301 acaagtcaaa ggtcacaact ccatccagat ctgtgtccag agttttgaaa aatcggaaca
      361 tggtctctag ccgcaccagg cagcaaacga aattgtcaaa gtcgaccgcc aggtcgggct
      421 ccgagtagcg ggtgatgatg agctcgtaca gcttcttgtt gagcttgaag cctgccgact
      481 caatggccat ccgcatctcg taggcactca tgctgcccga cttgtccagg tcaaacttcc
      541 ggaagatgga caggtaattc cggatgcggt tccacaggat gttgaactcc accaggccca
      601 gcttcccatt gccatcacga tccatgaggt tcaccatgct gcggcacgac tctaggctga
      661 agcccttggt ccgcaggtct ttgtgtttgc tgatgatcct attgaggatt gtccgcaact
      721 ccttcacgct gatctccatg tcctcccctg ccagctgcct gaagagggcc ttgaagttct
      781 cgtcaatctc ctcttctgag agcacttgct catcggggag attggcctgg atctggtcat
      841 ccagctccac agtcccagca ctcttctctg agaagaagcg cagcacgaag tcgccctcct
      901 tgttgggctc gaaggtggag ggcaccacca catactcccc gggtggcagg cggaagcggg
      961 tgctgacctc tcgcaggttg atgaactgct ctgagcgcgc ccgagacgca ttggccagga
     1021 agaagtcacg cttcaagtgt acggccggct ggcccaccag ct
//
