LOCUS       sequence-100120-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tet2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Wang Y, Zhang Y
  TITLE     Regulation of TET protein stability by calpains.
  JOURNAL   Cell Rep. 2014 Jan 30;6(2):278-84. doi: 
            10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.
  PUBMED    24412366
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.12.031"; journalName: "Cell Rep"; date: 
            "2014-01-30- 30"; volume: "6"; issue: "2"; pages: "278-84"
COMMENT     Sequence Label: pcDNA3-Tet2
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          118..690
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             736..759
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gctagagtcg atcctgagaa cttcagggtg
      121 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
      181 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      241 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      301 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
      361 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
      421 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
      481 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
      541 attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga
      601 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
      661 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt ttgtgctgtc
      721 gaccccaagc ttatggacta caaagacgat gacgacaagg gatccactag taacggccgc
      781 cagtgtgctg gaattcgccc tttagaacag gacagaacca cccatgctga gggcaccaga
      841 ctgagtccat tcctgatagc accaccttct cccatcagcc atacagaacc tctggctgtc
      901 aaactccaga atggaagccc gttagcagag agacctcatc cagaagtaaa tggagacacc
      961 aagtggcaat cttcccaaag ctgttatgga atatcccaca tgaaaggcag ccagagcagt
     1021 catgagagtc cacatgaaga cagagggtat tccaggtgct tacagaatgg agggataaa
//
