LOCUS       sequence-100129-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange 
            dsRed with flip-out conditional cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-0-FRT-2xTY1-FRT-V5
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    187..234
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    300..347
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             349..390
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    578..647
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cgtactccac ctcacccatc tggtccatca tgatggtcga ctctagaagt cgatccaaca
       61 tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga actaatttta
      121 atcaaccgat ttatctctct tccgcaggtg ggaggttccg gtggaagcgg aggtagcggc
      181 ggatcggaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaggtc
      241 cacactaatc aagaccccct ggatgccgag gtgcacacca accaggaccc tctggaccgg
      301 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcggg caagcccatc
      361 cccaaccccc tgctgggcct ggatagcacc agatccggag gtagcggtgg aagcggaggt
      421 tccggcgagg taagttattg aacaatggca tcaaatgcct tcatcatcac taccctttag
      481 cccttaagac cccacaatga ccttacccac tcagagaaaa aagtaaatat gaaagcccat
      541 ttgaacttct aagcttgtcg accatcatga tggaccagat gggtgaggtg gagtacgcgc
      601 ccggggagcc caagggcacg ccctggcacc cgcaccgcgg cttcgagacc gtgacctaca
      661 tcatgcatgg tacccagctt ttg
//
