LOCUS       sequence-100132-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange 
            dsRed with 2xTY1 and V5 tags cassette .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..104
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             370..411
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    599..668
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cgaattggag ctcgctagcg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg
       61 gcgtgccctt gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatgg
      121 tcgactctag aagtcgatcc aacatggcga cttgtcccat ccccggcatg tttaaatata
      181 ctaattattc ttgaactaat tttaatcaac cgatttatct ctcttccgca ggtgggaggt
      241 tccggtggaa gcggaggtag cggcggatcg gaggtccaca ctaatcaaga ccccctggat
      301 gccgaggtgc acaccaacca ggaccctctg gacggaggtt ccggtggaag cggaggtagc
      361 ggcggatcgg gcaagcccat ccccaacccc ctgctgggcc tggatagcac cagatccgga
      421 ggtagcggtg gaagcggagg ttccggcgag gtaagttatt gaacaatggc atcaaatgcc
      481 ttcatcatca ctacccttta gcccttaaga ccccacaatg accttaccca ctcagagaaa
      541 aaagtaaata tgaaagccca tttgaacttc taagcttgtc gaccatcatg atggaccaga
      601 tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac ccgcaccgcg
      661 gcttcgagac cgtgacctac atc
//
