LOCUS       sequence-100135-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange 
            dsRed with flip-out conditional cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-2-FRT-2xTY1-FRT-V5
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    190..237
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    303..350
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             352..393
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    580..649
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cgcgtactcc acctcaccca tctggtccat catgatggtc gactctagaa gtcgatccaa
       61 catggcgact tgtcccatcc ccggcatgtt taaatatact aattattctt gaactaattt
      121 taatcaaccg atttatctct cttccgcagg tcgggaggtt ccggtggaag cggaggtagc
      181 ggcggatcgg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcgag
      241 gtccacacta atcaagaccc cctggatgcc gaggtgcaca ccaaccagga ccctctggac
      301 cggaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc gggcaagccc
      361 atccccaacc ccctgctggg cctggatagc accagatccg gaggtagcgg tggaagcgga
      421 ggttccggca ggtaagttat tgaacaatgg catcaaatgc cttcatcatc actacccttt
      481 agcccttaag accccacaat gaccttaccc actcagagaa aaaagtaaat atgaaagccc
      541 atttgaactt ctaagcttgt cgaccatcat gatggaccag atgggtgagg tggagtacgc
      601 gcccggggag cccaagggca cgccctggca cccgcaccgc ggcttcgaga ccgtgaccta
      661 catcatgcat ggtacccagc
//
