LOCUS       sequence-100136-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange 
            dsRed with 2xTY1 and V5 tags cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Zhang X, Koolhaas WH, Schnorrer F
  TITLE     A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient 
            Genome Engineering in Drosophila.
  JOURNAL   G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979.
  PUBMED    25324299
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 
            10.1534/g3.114.013979."
COMMENT     Sequence Label: pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..98
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             365..406
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    593..662
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 ggagctcgct agcgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc
       61 ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg atggtcgact
      121 ctagaagtcg atccaacatg gcgacttgtc ccatccccgg catgtttaaa tatactaatt
      181 attcttgaac taattttaat caaccgattt atctctcttc cgcaggtcgg gaggttccgg
      241 tggaagcgga ggtagcggcg gatcggaggt ccacactaat caagaccccc tggatgccga
      301 ggtgcacacc aaccaggacc ctctggacgg aggttccggt ggaagcggag gtagcggcgg
      361 atcgggcaag cccatcccca accccctgct gggcctggat agcaccagat ccggaggtag
      421 cggtggaagc ggaggttccg gcaggtaagt tattgaacaa tggcatcaaa tgccttcatc
      481 atcactaccc tttagccctt aagaccccac aatgacctta cccactcaga gaaaaaagta
      541 aatatgaaag cccatttgaa cttctaagct tgtcgaccat catgatggac cagatgggtg
      601 aggtggagta cgcgcccggg gagcccaagg gcacgccctg gcacccgcac cgcggcttcg
      661 agaccgtgac ctacatc
//
