LOCUS       sequence-100149-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Sauer B
  TITLE     Manipulation of transgenes by site-specific recombination: use of 
            Cre recombinase.
  JOURNAL   Methods Enzymol. 1993;225:890-900. doi: 
            10.1016/0076-6879(93)25056-8.
  PUBMED    8231893
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1993"; volume: "225:890-900. doi"; pages: " 
            10.1016/0076-6879(93)25056-8"
COMMENT     Sequence Label: pBS302
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..100)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          634..699
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             829..849
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaacgcgcga ggcagcctcg aagcggccgc aatggccagt actagtgaac ctcttcgagg
       61 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccggatcctc
      121 ggggacacca aatatggcga tctcggcctt ttcgtttctt ggagctggga catgtttgcc
      181 atcgatccat ctaccaccag aacggccgtt agatctgctg ccaccgttgt ttccaccgaa
      241 gaaaccaccg ttgccgtaac caccacgacg gttgttgcta aagaagctgc caccgccacg
      301 gccaccgttg tagccgccgt tgttgttatt gtagttgcta ctgttatttc tggcacttct
      361 tggttttcct cttaagtgag gaggaacata accattctcg ttgttgtcgt tgatgcttaa
      421 attttgcact tgttcgctca gttcagccat aatatgaaat gcttttcttg ttgttcttac
      481 ggaataccac ttgccaccta tcaccacaac taactttttc ccgttcctcc atctctttta
      541 tatttttttt ctcgagggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
      601 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
      661 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
      721 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
      781 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
      841 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
      901 gtgtttagta atagaactct
//
