LOCUS       sequence-100151-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Sauer B
  TITLE     Manipulation of transgenes by site-specific recombination: use of 
            Cre recombinase.
  JOURNAL   Methods Enzymol. 1993;225:890-900. doi: 
            10.1016/0076-6879(93)25056-8.
  PUBMED    8231893
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1993"; volume: "225:890-900. doi"; pages: " 
            10.1016/0076-6879(93)25056-8"
COMMENT     Sequence Label: pBS302
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..121
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    171..204
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    246..380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(270..289)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     324..343
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(805..825)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acggcggccg
       61 cacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      121 ccctttcgtc ttcaagaatt ccatcaagct taggatccgg aacccttaat ataacttcgt
      181 ataatgtatg ctatacgaag ttattaggtc cctcgacctg cagcccaagc ttacttacca
      241 tgtcagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      301 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      361 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      421 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      481 atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa
      541 aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg
      601 tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac
      661 agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat
      721 agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc
      781 tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg
      841 agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc
      901 ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg gaatc
//
