LOCUS       sequence-100157-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector of rat Kit-2-1 guide RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Yoshimi K, Kaneko T, Voigt B, Mashimo T
  TITLE     Allele-specific genome editing and correction of disease-associated 
            phenotypes in rats using the CRISPR-Cas platform.
  JOURNAL   Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
  PUBMED    24967838
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-26"; volume: "5:4240. doi"; pages: " 
            10.1038/ncomms5240"
COMMENT     Sequence Label: pT7-gRNA:kit-2-1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(60..162)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(163..194)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     242..258
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     242..258
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        387..405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        426..501
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(683..700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(683..699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tccatgttgg aatttaatcg cggcttcaag caagacgttt cccgttgaat atggctcatt
       61 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
      121 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
      181 ggaggctttt gactttctgc tatggaggtc aggtatgatt taaatggtca gtattgagcc
      241 tcaggaaaca gctatgacat caagctgact agataatcta gctgatcgtg gaccgatcat
      301 acgtataatg ccgtaagatc acgggtcgca gcacagctcg cggtccagta gtgatcgaca
      361 ctgctcgatc cgctcgcacc gctagctaat acgactcact ataggctaac gttccagcgc
      421 tcgttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      481 aaagtggcac cgagtcggtg cttttaaaaa gcttggatcg acgagagcag cgcgactgga
      541 tctgtcgccc gtctcaaacg caaccctccg gcggtcgcat atcattcagg acgagcctca
      601 gactccagcg taactggact gcaatcaact cactggctca ccttccggtc cacgatcagc
      661 tagaatcaag ctgactagat aaactggccg tcgttttaca cgggtgggcc tttcttcggt
      721 agaaaatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      841 ttccgaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      901 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      961 cctgttacca gtggctgctg ccagtggcga ta
//
