LOCUS       sequence-100161-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to knockdown endogenous miR144 levels; lentiviral vector containing 
            8x copies of complementary miR-144-binding sites cloned 3' to GFP 
            under the control of a biPGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
  TITLE     MIR144 and MIR451 regulate human erythropoiesis via RAB14.
  JOURNAL   Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
  PUBMED    25312678
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Br J 
            Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164."
COMMENT     Sequence Label: miR-144 Trap
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..45)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             406..639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
       61 tcccgcggcc tgctgccggc tctagagaat tcagtacatc atctatactg tatagagaac
      121 ttagtacatc atctatactg taagagaact taagtacatc atctatactg tagaacttag
      181 agagtacatc atctatactg tacttagaga acagtacatc atctatactg taagagaact
      241 taagtacatc atctatactg tatagagaac ttagtacatc atctatactg tacttagaga
      301 acagtacatc atctatactg taaccggtga attcggtacc tttaagacca atgacttaca
      361 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
      421 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
      661 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgaga
//
