LOCUS       sequence-100167-       10881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector; TetON promoter driving human Ngn1;
            constitutive expression of Blasticidine selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana 
            NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
  TITLE     Rapid neurogenesis through transcriptional activation in human stem 
            cells.
  JOURNAL   Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
  PUBMED    25403753
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-17"; volume: "10(11):760. doi"; pages: " 
            10.15252/msb.20145508"
COMMENT     Sequence Label: pLV_TRET_hNgn1_UBC_Bla
FEATURES             Location/Qualifiers
     source          1..10881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..118
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             137..511
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    641..762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(678..697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     732..751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(811..827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(811..827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(824..846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    835..851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(859..865,866..883,884..889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    904..925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1042..1059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1213..1801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1293..1312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1972..2763,2764..2832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2595..2614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2833..2937)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3012..3031)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3203..3582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3584..3782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3800..3980
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4027..4152
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4645..4878
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5063..5107
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5405..5522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5598..5618
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5663..5681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5698..5716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5734..5752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5770..5788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5805..5823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5841..5859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5871..5909
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5906..5930
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5949..5973
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6700..6724)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6777..6818
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        6840..8051
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7888..7907
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      8081..8090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8087..8485
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8538..9126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8591..8611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9009..9020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9129..9145)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9130..9146)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9651..9831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9857..9874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9863..10087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10133..10561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10220..10239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10430..10451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10570..10590)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10575..10881,1..23)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10755..10881,1..9)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10817..10836
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
       61 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
      121 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
      181 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
      241 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
      301 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
      361 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
      421 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
      481 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
      541 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
      601 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
      661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
      781 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      841 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      901 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      961 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1021 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1621 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2101 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2281 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2821 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2881 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2941 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     3001 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     3061 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     3121 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     3181 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     3241 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     3481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     3541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     3601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     3661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     3721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     3781 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3961 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     4021 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     4081 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     4141 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     4201 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     4261 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     4321 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     4381 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     4441 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     4501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     4561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     4621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     4681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     4741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     4801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     4861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     4921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     4981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     5041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     5101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     5161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     5221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     5281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     5341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     5401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     5461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     5521 ttcgggttta ttacagggac agcagagatc cagtttggtt aattaagtat cgatgtcgac
     5581 gttaacgcta gtgatatcaa ctttgtatag aaaagttggc tccgaattcg cccttcaggt
     5641 ccgaggttct agacgagttt actccctatc agtgatagag aacgatgtcg agtttactcc
     5701 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     5761 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttatcccta tcagtgatag
     5821 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg
     5881 tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcaaagggcg
     5941 aattcgaccc aagtttgtac aaaaaagcag gctgacctgc caccatgcca gcccgccttg
     6001 agacctgcat ctccgacctc gactgcgcca gcagcagcgg cagtgaccta tccggcttcc
     6061 tcaccgacga ggaagactgt gccagactcc aacaggcagc ctccgcttcg gggccgcccg
     6121 cgccggcccg caggggcgcg cccaatatct cccgggcgtc tgaggttcca ggggcacagg
     6181 acgacgagca ggagaggcgg cggcgccgcg gccggacgcg ggtccgctcc gaggcgctgc
     6241 tgcactcgct gcgcaggagc cggcgcgtca aggccaacga tcgcgagcgc aaccgcatgc
     6301 acaacttgaa cgcggccctg gacgcactgc gcagcgtgct gccctcgttc cccgacgaca
     6361 ccaagctcac caaaatcgag acgctgcgct tcgcttacaa ctacatctgg gctctggccg
     6421 agacactgcg cctggcggat caagggctgc ccggaggcgg tgcccgggag cgcctcctgc
     6481 cgccgcagtg cgtcccctgc ctgcccggtc ccccaagccc cgccagcgac gcggagtcct
     6541 ggggctcagg tgccgccgcc gcctccccgc tctctgaccc cagtagccca gccgcctccg
     6601 aagacttcac ctaccgcccc ggcgaccctg ttttctcctt cccaagcctg cccaaagact
     6661 tgctccacac aacgccctgt ttcattcctt accactagta cccagctttc ttgtacaaag
     6721 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     6781 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtttt aagtgatctg
     6841 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     6901 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     6961 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     7021 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     7081 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     7141 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     7201 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     7261 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     7321 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     7381 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     7441 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     7501 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     7561 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     7621 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     7681 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     7741 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     7801 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     7861 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     7921 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     7981 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     8041 ttttgttaga cgaagcttgg gctgcaggtc gactctagat gccaccatgg ccaagccttt
     8101 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     8161 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     8221 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     8281 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     8341 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     8401 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     8461 ctctggttat gtgtgggagg gctaaggcta agcacaattc gagctcggta ccgcaattcg
     8521 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     8581 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8641 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8701 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8761 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8821 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8881 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8941 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9001 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     9061 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9121 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     9181 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     9241 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     9301 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9361 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     9421 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     9481 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     9541 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     9601 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     9661 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9721 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9781 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     9841 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9901 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9961 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    10021 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    10081 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
    10141 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10201 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10261 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10321 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10381 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10441 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10501 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10561 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10621 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10681 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10741 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    10861 agaagtagtg aggaggcttt t
//
