LOCUS       sequence-100178-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Hadjantonakis AK, Macmaster S, Nagy A
  TITLE     Embryonic stem cells and mice expressing different GFP variants for 
            multiple non-invasive reporter usage within a single animal.
  JOURNAL   BMC Biotechnol. 2002 Jun 11;2:11.
  PUBMED    12079497
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2002-06-11"; volume: "2"; pages: "11"
COMMENT     Sequence Label: pCAG:ECFP
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(68..781,782..784,785..787))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPGGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVHITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(113..134)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     462..481
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     721..742
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(821..840)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(902..924)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
       61 tgaattctta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      121 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      181 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      241 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttggcct
      301 tgatgccgtt cttctgcttg tcggcggtga tatggacgtt gtggctgatg tagttgtact
      361 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      421 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      481 ccttgaagaa gatggtgcgc tcctggacgt agcctccggg catggcggac ttgaagaagt
      541 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccccag gtcagggtgg
      601 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      661 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      721 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      781 tcaccatggt ggcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc
      841 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc
      901 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc
      961 cccgcggaca gccccgaggc tggagagg
//
