LOCUS       sequence-100179-       11257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  const. CenpB-GFP-Halo + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB221
FEATURES             Location/Qualifiers
     source          1..11257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..100
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    312..345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             350..949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(350..369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     846..866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1085..1102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1091..1202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1347..1650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1652..1929
                     /label=chicken beta-actin promoter
     intron          1930..2946
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2870..2892
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2954..2973
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4838..4847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4844..4846,4847..4849,4850..5560)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4844..4846,4847..4849,4850..5560)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4889..4910)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5150..5169)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5497..5518
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5579..6466
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    6499..7072
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6522..7074
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6666..6683)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6893..6912
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7074..7781
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7219..7237)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7487..7506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7800..8273
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             8274..8303
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8404..8423)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8469..8524
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8523..8542)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    8885..8918
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    8931..9052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8968..8987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9022..9041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9071..9091)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9071..9089)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9110..9126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9110..9126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9123..9145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9134..9150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9158..9164,9165..9182,9183..9188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9203..9224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9341..9358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9512..10100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9592..9611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10271..11062,11063..11131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10894..10913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11132..11236)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttaata aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
       61 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
      121 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
      181 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
      241 gtcggggcgg caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca
      301 ttcgagaata aataacttcg tataaagtat cctatacgaa gttatcacca tgaccgagta
      361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
      421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
      481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
      541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
      601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
      661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
      721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
      781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
      841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
      901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
      961 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccgacc
     1021 cgggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag agctcgctga
     1081 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1141 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1201 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1261 ggggaggatt gggaagacaa tagcactagt tattaatagt aatcaattac ggggtcatta
     1321 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     1381 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1441 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1501 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1561 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1621 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     1681 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     1741 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1801 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1861 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1921 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1981 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2041 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     2101 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     2161 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     2221 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     2281 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     2341 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     2401 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     2461 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     2521 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2581 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2641 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2701 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2761 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2821 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2881 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2941 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     3001 ctcgaccacc ggcgcgccac cggtcaccat gggccccaag aggcgacagc tgacgttccg
     3061 ggagaagtca cggatcatcc aggaggtgga ggagaatccg gacctgcgca agggcgagat
     3121 cgcgcggcgc ttcaacatcc cgccgtccac gctgagcacg atcctgaaga acaagcgcgc
     3181 catcctggcg tcggagcgca agtacggggt ggcctccacc tgccgcaaga ccaacaagct
     3241 gtctccctac gacaagctcg agggcttgct catcgcctgg ttccagcaga tccgcgccgc
     3301 cggcctgccg gtcaagggca tcatcctcaa ggagaaggcg ctgcgcatag ccgaggagct
     3361 gggcatggac gacttcaccg cctccaacgg ctggctggac cgcttccgcc ggcgccacgg
     3421 cgtggtgtcc tgcagcggcg tggcccgcgc ccgcgcgcga aacgctgccc cccgcacccc
     3481 ggcggcgcct gccagtccgg ccgcggtgcc ctcggagggc agtggcggga gcactactgg
     3541 ttggcgcgct cgggaggagc agccgccgtc ggtggccgag ggctacgcct cgcaggacgt
     3601 gttcagcgcc accgagacca gtctatggta cgacttcctg cccgaccagg ccgcggggct
     3661 gtgcggaggc gacggacggc cgcgtcaagc cacccagcgc ctgagcgtcc tgctatgcgc
     3721 caatgccgac ggcagcgaga agctgccccc gctggtggcc ggcaagtcgg ccaagccccg
     3781 cgcaggccaa gccggcctgc cctgcgacta caccgccaac tccaagggtg gtgtcaccac
     3841 ccaggccctg gccaagtact tgaaggcctt ggacacccga atggctgcag agtctcgccg
     3901 ggtcctgctg ttggccggcc gcttggctgc ccagtccttg gacacctcgg gcctgcggca
     3961 tgtgcagctg gccttcttcc ctcccggcac cgtgcatccg ctggagaggg gagtggtcca
     4021 gcaggtgaag ggccactacc gccaggccat gctgctcaag gccatggccg cgctagaggg
     4081 ccaggatccc tcaggcctgc agctgggtct cacggaggcc ctgcactttg tggctgccgc
     4141 ctggcaggca gtggagcctt cggacatagc cgcctgcttt cgtgaggctg gctttggggg
     4201 tggccctaat gccaccatca ccacttccct caagagtgag ggagaggaag aggaggagga
     4261 ggaggaagaa gaggaggagg aagagggtga aggagaggaa gaggaggagg aaggggagga
     4321 ggaggaggag gaaggggggg aaggagagga attgggggag gaagaggagg tggaggagga
     4381 gggtgatgtt gatagtgatg aagaagagga ggaagatgag gagagctcct cggagggctt
     4441 ggaggctgag gactgggccc agggagtagt ggaggccggt ggcagcttcg gggcttatgg
     4501 tgcccaggag gaagcccagt gccctactct gcatttcctg gaaggtgggg aggactctga
     4561 ttcagacagt gaggaagagg acgatgagga agaggatgat gaagatgaag acgacgatga
     4621 tgatgaggag gatggtgatg aggtgcctgt acccagcttt ggggaggcca tggcttactt
     4681 tgccatggtc aagaggtacc tgacctcctt ccccattgat gaccgcgtgc agagccacat
     4741 cctccacttg gaacacgatc tggttcatgt gaccaggaag aaccacgcca ggcaggcggg
     4801 agttcgaggt cttggacatc aaagcgggct agcagtcgcc accatggtga gcaagggcga
     4861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     5041 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     5161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5521 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggtgaaa cgcgtggtgc
     5581 agaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat
     5641 gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc
     5701 gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat
     5761 tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga
     5821 cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct
     5881 ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg
     5941 cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc
     6001 agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat
     6061 catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac
     6121 tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact
     6181 gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt
     6241 cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac
     6301 cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg
     6361 caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat
     6421 cggcagcgag atcgcgcgct ggctgtcgac gctcgagatt tccggctagc cggtctgcag
     6481 cctaggctta aggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     6541 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6601 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     6661 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     6721 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     6781 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     6841 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     6901 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     6961 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     7021 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggtga
     7081 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     7141 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     7201 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     7261 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     7321 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     7381 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     7441 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     7501 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     7561 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     7621 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     7681 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     7741 agggccgcca ctccaccggc ggcatggacg agctgtataa ggccggcctg gagtctagaa
     7801 tcagtctgat tgcggcgtta gcggtagatc gcgttatcgg catggaaaac gccatgccgt
     7861 ggaacctgcc tgccgatctc gcctggttta aacgcaacac cttaaataaa cccgtgatta
     7921 tgggccgcca tacctgggaa tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc
     7981 tcagcagtca accgggtacg gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca
     8041 tcgcggcgtg tggtgacgta ccagaaatca tggtgattgg cggcggtcgc gtttatgaac
     8101 agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg
     8161 acacccattt cccggattac gagccggatg actgggaatc ggtattcagc gaattccacg
     8221 atgctgatgc gcagaactct cacagctatt gctttgagat tctggagcgg cgggaacaaa
     8281 aactcatctc agaagaggat ctttaattaa gcgctcgagg cggccgcctc ctcaggtgca
     8341 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     8401 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     8461 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     8521 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     8581 ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag
     8641 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     8701 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     8761 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     8821 tagctgtccc tcttctctta tgaagatccc tcgacctgag cgtcgagcta gcatatggat
     8881 ccatataact tcgtataatg tatgctatac gaagttatta cgataccgtc aacttgttta
     8941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taggtaccca
     9061 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     9121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11221 aaataggggt tccgcgcaca tttccccgaa aagtgcc
//
