LOCUS       sequence-10018-e        5836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark SG, Ron D
  TITLE     A survival pathway for Caenorhabditis elegans with a blocked 
            unfolded protein response.
  JOURNAL   J Cell Biol. 2002 Aug 19. 158(4):639-46.
  PUBMED    12186849
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Aug 19. 158(4):639-46."
COMMENT     Sequence Label: SREC 1: abu-1::gfp
FEATURES             Location/Qualifiers
     source          1..5836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1506..1534)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2282..2302
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3276..3298)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3398..3417
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3495..3513)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3581..3685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3686..3754,3755..4546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3904..3923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4717..5305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5206..5225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5459..5476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5593..5614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5629..5634,5635..5652,5653..5659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5667..5683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5672..5694
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5691..5707
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5691..5707
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcagct ggagtctgga caacacagga ttgttcacag gctggcatgc
       61 aggatggttg acattgaact ggagctggag tagttggagt ataacaggaa gaagtctgac
      121 agctttgttg gcatgtagat tgacattgac taacagatgg agcagattga cattggttct
      181 ggcaagactg ctgacaagct ggggcacatt gagaagcttg aatttggtag gtttgttgct
      241 gaactggttg ggcacatgaa caggatttct ggactggagc agtgttctga caggaacaac
      301 tgtactgttg tggttgagta taggtggttc gttggcaaga gcaacttggt tgaactcttg
      361 gagcacagcc acaggattgg cgtttatcgc gaattgttgt tgcctggaaa tgatttttgt
      421 gaaatttgac tgaaatcgtg gtcaatcgtg gtcgactgaa atcgtggaat atataatctt
      481 atgtctgcaa gcaatgctca agaaaaacaa atatttgtta aatttgggta cttagccgat
      541 ttttttggat cgccgctcag ccctgttcaa caactattta aaatgaacat ttggctcaac
      601 aatactatta gatggccatt atacctcaat aaggaaacag cttgaaacaa gaagagctgc
      661 aattgcgaag aagcgcatga tacggttcga aaggattgga acagatgatt ttctctagtt
      721 tccaggcctt ttatactcac actgtatggt taaccaaaga acataaaccc tgaattcccg
      781 cctatttatc ttaatgtaaa aaatgatgcg gaggagatca acactttgat ggaagcttag
      841 tgagcaaaat gataagtgcg cgaacaggga aaaagtagtt ttatttatgt cgtaatgagt
      901 ggtcttgaaa gtcgataaac ttaaagtgta cttgtgaaat caaatgttca tctcaaaatg
      961 taaaagacaa atatttacca gcagtaccta aaaaggtaaa cgataacttt ggtacatata
     1021 gttaagcaga aactcgtttc ggtcaaacac acaccgtatc agcacattcc attcgacgga
     1081 acgcgcaccc aacctttttt caatctttgc aactctccgt gctcccaaaa aatagtgtgt
     1141 aaaaccatga gacggcacac gtgttcttcg gatcatttgc tatgcctcca ttgctatgtt
     1201 tgcgctcgtc atggagccgg ttgatggata gagtctgata agtgggaagc aactttccgg
     1261 tatgtgttcg atggttagtg aaaaagtgta ggagaaggtt ggtataaaag tctgaaaggt
     1321 tgacaaaagg acatcagttg ccgtctagca gctcttcaag catattaaca atggatcccc
     1381 gggattggcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac atagaacatt
     1441 ttcaggagga cccttggagg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
     1501 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     1561 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1621 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
     1681 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
     1741 cgagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt
     1801 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
     1861 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
     1921 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1981 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     2041 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
     2101 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     2161 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     2221 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     2281 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     2341 aatagcattc gtagaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg
     2401 tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac
     2461 gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac
     2521 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     2581 caaaaaaact tcttcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat
     2641 gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc
     2701 tccctccccc cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt
     2761 cttatgtttt ttttacttct gataaatttt ttttgaaaca tcatagaaaa aaccgcacac
     2821 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     2881 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     2941 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttgggggt
     3001 ttcccctatt gtttgtcaag agtttcgagg acggcgtttt tcttgctaaa atcacaagta
     3061 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
     3121 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga
     3181 cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc gtacgggccc
     3241 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3301 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     3361 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     3421 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3481 aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3541 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3661 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3721 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3781 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3841 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3901 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3961 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4021 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4081 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4141 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4201 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4261 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4321 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4381 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4441 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4501 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4561 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4621 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4681 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4741 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4801 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4861 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4921 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4981 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5041 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5101 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5161 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5221 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5281 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5341 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5401 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5461 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5521 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5581 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5641 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5701 ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct
     5761 catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac
     5821 aacttggaaa tgaaat
//
