LOCUS       sequence-10019-e        7220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Urano F, Calfon M, Yoneda T, Yun C, Kiraly M, Clark SG, Ron D
  TITLE     A survival pathway for Caenorhabditis elegans with a blocked 
            unfolded protein response.
  JOURNAL   J Cell Biol. 2002 Aug 19. 158(4):639-46.
  PUBMED    12186849
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Aug 19. 158(4):639-46."
COMMENT     Sequence Label: SREC 2: abu-1::abu-1::gfp
FEATURES             Location/Qualifiers
     source          1..7220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2890..2918)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3666..3686
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4660..4682)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4782..4801
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4879..4897)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4965..5069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5070..5138,5139..5930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5288..5307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6101..6689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6590..6609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6843..6860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6977..6998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7013..7018,7019..7036,7037..7043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7051..7067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7056..7078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7075..7091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7075..7091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcagct ggagtctgga caacacagga ttgttcacag gctggcatgc
       61 aggatggttg acattgaact ggagctggag tagttggagt ataacaggaa gaagtctgac
      121 agctttgttg gcatgtagat tgacattgac taacagatgg agcagattga cattggttct
      181 ggcaagactg ctgacaagct ggggcacatt gagaagcttg aatttggtag gtttgttgct
      241 gaactggttg ggcacatgaa caggatttct ggactggagc agtgttctga caggaacaac
      301 tgtactgttg tggttgagta taggtggttc gttggcaaga gcaacttggt tgaactcttg
      361 gagcacagcc acaggattgg cgtttatcgc gaattgttgt tgcctggaaa tgatttttgt
      421 gaaatttgac tgaaatcgtg gtcaatcgtg gtcgactgaa atcgtggaat atataatctt
      481 atgtctgcaa gcaatgctca agaaaaacaa atatttgtta aatttgggta cttagccgat
      541 ttttttggat cgccgctcag ccctgttcaa caactattta aaatgaacat ttggctcaac
      601 aatactatta gatggccatt atacctcaat aaggaaacag cttgaaacaa gaagagctgc
      661 aattgcgaag aagcgcatga tacggttcga aaggattgga acagatgatt ttctctagtt
      721 tccaggcctt ttatactcac actgtatggt taaccaaaga acataaaccc tgaattcccg
      781 cctatttatc ttaatgtaaa aaatgatgcg gaggagatca acactttgat ggaagcttag
      841 tgagcaaaat gataagtgcg cgaacaggga aaaagtagtt ttatttatgt cgtaatgagt
      901 ggtcttgaaa gtcgataaac ttaaagtgta cttgtgaaat caaatgttca tctcaaaatg
      961 taaaagacaa atatttacca gcagtaccta aaaaggtaaa cgataacttt ggtacatata
     1021 gttaagcaga aactcgtttc ggtcaaacac acaccgtatc agcacattcc attcgacgga
     1081 acgcgcaccc aacctttttt caatctttgc aactctccgt gctcccaaaa aatagtgtgt
     1141 aaaaccatga gacggcacac gtgttcttcg gatcatttgc tatgcctcca ttgctatgtt
     1201 tgcgctcgtc atggagccgg ttgatggata gagtctgata agtgggaagc aactttccgg
     1261 tatgtgttcg atggttagtg aaaaagtgta ggagaaggtt ggtataaaag tctgaaaggt
     1321 tgacaaaagg acatcagttg ccgtctagca gctcttcaag catattaaca atggatcccc
     1381 gggctgcagg aattcagatg cggatattcc ttgtctctgc agctcttatt gcttctagcg
     1441 ttctacttgc cgaagtacgt ttcttatcac ttacgaatca ctgttgtaaa atacattaat
     1501 aattatattt atcttacaat accatataat tttcaggcaa caacaattcg cgataaacgc
     1561 caatcctgtg gctgtgctcc aagagttcaa ccaagttgct cttgccaacg aaccacctat
     1621 actcaaccac aacagtacag ttgttcctgt cagaacactg ctccagtcca gaaatcctgt
     1681 tcatgtgccc aaccagttca gcaacaaacc taccaaattc aagcttctca atgtgcccca
     1741 gcttgtcagc agtcttgcca gaaccaatgt caatctgctc catctgttag tcaatgtcaa
     1801 tctacatgcc aacaaagctg tcagacttct tcctgttata ctccaactac tccagctcca
     1861 gttcaatgtc aaccatcctg catgccagcc tgtgaacaat cctgtgttgt ccagactcca
     1921 gctccagttc aatgcgtgcc acaatgccaa caacagtgcc aacagcaatg tgttcaaact
     1981 caaccaattc aacaatgtca gccacaatgt cagcaacaat gtgttcaaca atgtgctcca
     2041 accactactg ccgctccaca aataatcaag attaacatgg aaatctctgc tcagtgtgtt
     2101 cctcaatgcc agcaatcatg ccaacaacaa tgtgttcaac aacaagttcc agcccaacag
     2161 tgcaatcagc aatgcaccca gcaatgccag accacctgcc agcaagcagt tccacaatgc
     2221 caacaacagt gtgctccaca atgccaacaa ccatcagctc cacagtgcca gcagtgccag
     2281 aatacctgcc aacaagctgc tccagtttgt cagcaacagt gtgctccaca atgtcaacag
     2341 caatctgcac cagcttgcca acaatgtcag acttcgtgcc aacaaactca acagtgccaa
     2401 caacaatgta ctccacagtg ccagcaacca tctgctccac aatgccagca atgccaatct
     2461 gcatgccaag ctccagttgc cactactgct gctccacaag ttgtcacaat cattctggaa
     2521 gcttcagtct cccaatctgc acaatgtgaa ccacaatgcc agcaatcatg ccaacagcaa
     2581 tgcgttcagc agcaacaacc aatgcaacaa tgtgctccag cttgcaccca atcttgctct
     2641 caatcctgtt ctgctgctca accagcccag atgccatgcc agacacaatc agtcaactcc
     2701 tgctcgtgcc agcaaaacta ttctccatgt ggaaatggac agtgttgcaa gagaaaggat
     2761 ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa
     2821 cattttcagg aggacccttg gagggtaccg gtagaaaaaa tgagtaaagg agaagaactt
     2881 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     2941 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     3001 tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac
     3061 cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc
     3121 ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
     3181 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag
     3241 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag
     3301 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     3361 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     3421 gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaactaac
     3481 taatctgatt taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt
     3541 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     3601 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     3661 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     3721 tacaaatagc attcgtagaa ttccaactga gcgccggtcg ctaccattac caacttgtct
     3781 ggtgtcaaaa ataatagggg ccgctgtcat cagagtaagt ttaaactgag ttctactaac
     3841 taacgagtaa tatttaaatt ttcagcatct cgcgcccgtg cctctgactt ctaagtccaa
     3901 ttactcttca acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta
     3961 ttatcaaaaa aacttcttct taatttcttt gttttttagc ttcttttaag tcacctctaa
     4021 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     4081 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     4141 acttcttatg ttttttttac ttctgataaa ttttttttga aacatcatag aaaaaaccgc
     4201 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     4261 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     4321 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     4381 gggtttcccc tattgtttgt caagagtttc gaggacggcg tttttcttgc taaaatcaca
     4441 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     4501 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     4561 atgacagaat acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg
     4621 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4681 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4741 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4861 catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4921 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4981 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5041 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5101 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5161 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5221 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5281 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5341 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5401 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5461 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5521 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5581 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5641 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5701 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5761 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5821 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5881 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5941 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6001 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6061 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6121 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6181 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6241 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6301 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6361 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6421 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6481 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6541 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6601 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6661 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6721 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6781 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6841 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6901 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6961 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7021 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7081 acagctatga ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta
     7141 ttctcattta aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc
     7201 taacaacttg gaaatgaaat
//
