LOCUS       sequence-100192-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Nowotschin S, Hadjantonakis AK
  TITLE     Use of KikGR a photoconvertible green-to-red fluorescent protein for
            cell labeling and lineage analysis in ES cells and mouse embryos.
  JOURNAL   BMC Dev Biol. 2009 Sep 9;9:49.
  PUBMED    19740427
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-09-9"; volume: "9"; pages: "49"
COMMENT     Sequence Label: pCAG:Kaede
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             64..744
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MVSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVV
                     KEGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIA
                     TNDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALL
                     LKGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLP
                     DNVK"
     primer_bind     complement(831..850)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    896..951
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(950..969)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
       61 cggatggtga gtctgattaa accagaaatg aagatcaagc tgcttatgga aggcaatgta
      121 aacgggcacc agtttgttat tgagggagat ggaaaaggcc atccttttga gggaaaacag
      181 agtatggacc ttgtagtcaa agaaggcgca cctctccctt ttgcctacga tatcttgaca
      241 acagcattcc attatggtaa cagggttttt gctaaatacc cagaccatat accagactac
      301 ttcaagcagt cgtttcccaa agggttttct tgggagcgaa gcctgatgtt cgaggacggg
      361 ggcgtttgca tcgctacaaa tgacataaca ctgaaaggag acactttttt taacaaagtt
      421 cgatttgatg gcgtaaactt tcccccaaat ggtcctgtta tgcagaagaa gactctgaaa
      481 tgggaggcat ccactgagaa aatgtatttg cgtgatggag tgttgacggg cgatattacc
      541 atggctctgc tgcttaaagg agatgtccat taccgatgtg acttcagaac tacttacaaa
      601 tctaggcagg agggtgtcaa gttgccagga tatcactttg tcgatcactg catcagcata
      661 ttgaggcatg acaaagacta caacgaggtt aagctgtatg agcatgctgt tgcccattct
      721 ggattgccgg acaacgtcaa gtaaccggaa ttcactcctc aggtgcaggc tgcctatcag
      781 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
      841 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
      901 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
      961 acatatggga gggcaaatca tttaaaacat cagaatgag
//
