LOCUS       sequence-100193-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Nowotschin S, Hadjantonakis AK
  TITLE     Use of KikGR a photoconvertible green-to-red fluorescent protein for
            cell labeling and lineage analysis in ES cells and mouse embryos.
  JOURNAL   BMC Dev Biol. 2009 Sep 9;9:49.
  PUBMED    19740427
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-09-9"; volume: "9"; pages: "49"
COMMENT     Sequence Label: pCAG:Kaede
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..755)
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MVSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVV
                     KEGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIA
                     TNDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALL
                     LKGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLP
                     DNVK"
     primer_bind     complement(786..805)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(867..889)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga
       61 ggagtgaatt ccggttactt gacgttgtcc ggcaatccag aatgggcaac agcatgctca
      121 tacagcttaa cctcgttgta gtctttgtca tgcctcaata tgctgatgca gtgatcgaca
      181 aagtgatatc ctggcaactt gacaccctcc tgcctagatt tgtaagtagt tctgaagtca
      241 catcggtaat ggacatctcc tttaagcagc agagccatgg taatatcgcc cgtcaacact
      301 ccatcacgca aatacatttt ctcagtggat gcctcccatt tcagagtctt cttctgcata
      361 acaggaccat ttgggggaaa gtttacgcca tcaaatcgaa ctttgttaaa aaaagtgtct
      421 cctttcagtg ttatgtcatt tgtagcgatg caaacgcccc cgtcctcgaa catcaggctt
      481 cgctcccaag aaaacccttt gggaaacgac tgcttgaagt agtctggtat atggtctggg
      541 tatttagcaa aaaccctgtt accataatgg aatgctgttg tcaagatatc gtaggcaaaa
      601 gggagaggtg cgccttcttt gactacaagg tccatactct gttttccctc aaaaggatgg
      661 ccttttccat ctccctcaat aacaaactgg tgcccgttta cattgccttc cataagcagc
      721 ttgatcttca tttctggttt aatcagactc accatccgga attctttgcc aaaatgatga
      781 gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca
      841 tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc
      901 cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg
      961 ggacggcgg
//
