LOCUS       sequence-100201-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector of rat Kit-2-2 guide RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Yoshimi K, Kaneko T, Voigt B, Mashimo T
  TITLE     Allele-specific genome editing and correction of disease-associated 
            phenotypes in rats using the CRISPR-Cas platform.
  JOURNAL   Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
  PUBMED    24967838
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-06-26"; volume: "5:4240. doi"; pages: " 
            10.1038/ncomms5240"
COMMENT     Sequence Label: pT7-gRNA:kit-2-2
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..66
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     50..66
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(248..323)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(343..362)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(344..362)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(491..507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(491..507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      555..586
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        587..689
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(763..782)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg taaaacgacg
       61 gccagtttat ctagtcagct tgattctagc tgatcgtgga ccggaaggtg agccagtgag
      121 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat gcgaccgccg
      181 gagggttgcg tttgagacgg gcgacagatc cagtcgcgct gctctcgtcg atccaagctt
      241 tttaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      301 cttgctattt ctagctctaa aacccgtaag atgacatctt gaccctatag tgagtcgtat
      361 tagctagcgg tgcgagcgga tcgagcagtg tcgatcacta ctggaccgcg agctgtgctg
      421 cgacccgtga tcttacggca ttatacgtat gatcggtcca cgatcagcta gattatctag
      481 tcagcttgat gtcatagctg tttcctgagg ctcaatactg accatttaaa tcatacctga
      541 cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa
      601 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
      661 cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct
      721 tgaagccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
      781 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
      841 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
      901 ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
      961 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc cag
//
