LOCUS       sequence-100204-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses DLX2 under the
            EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Victor MB, Richner M, Hermanstyne TO, Ransdell JL, Sobieski C, Deng 
            PY, Klyachko VA, Nerbonne JM, Yoo AS
  TITLE     Generation of Human Striatal Neurons by MicroRNA-Dependent Direct 
            Conversion of Fibroblasts.
  JOURNAL   Neuron. 2014 Oct 22;84(2):311-23. doi: 10.1016/j.neuron.2014.10.016.
            Epub 2014 Oct 22.
  PUBMED    25374357
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.10.016"; journalName: "Neuron"; date: 
            "2014-10-22- 22"; volume: "84"; issue: "2"; pages: "311-23"
COMMENT     Sequence Label: phDLX2-N174
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(55..554)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(103..122)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     496..519
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(637..657)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatgg tcctaggaag cttgggctgc aggtcgaaag
       61 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
      121 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
      181 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
      241 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
      301 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
      361 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
      421 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
      481 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
      541 cgcctcccct acccggtaga attcacgcgt tcgcgatcta gagccggcgc ggccttagaa
      601 aatcgtcccc gcgctcaccg gggcgccccc gccgccgtga tggtggtggt ggtgatgcgg
      661 ctgcggggtc tgagtggggt gcagcagcgg cgccgtggcc tgc
//
