LOCUS       sequence-100210-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zhou BP, Liao Y, Xia W, Spohn B, Lee MH, Hung MC
  TITLE     Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced 
            phosphorylation in HER-2/neu-overexpressing cells.
  JOURNAL   Nat Cell Biol. 2001 Mar . 3(3):245-52.
  PUBMED    11231573
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2001 Mar . 3(3):245-52."
COMMENT     Sequence Label: Flag p21 WT
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             74..96
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             151..645
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
ORIGIN
        1 accgtcagaa ttcagatctg gtaccacgcg tatcgataag cttgcatgcc tgcaggtcga
       61 ctctagaaat aattttgttt aactttaaga aggagatata ccatggacta caaagacgat
      121 gacgataaag caagaagagc atctgtgcat atgtcagaac cggctgggga tgtccgtcag
      181 aacccatgcg gcagcaaggc ctgccgccgc ctcttcggcc cagtggacag cgagcagctg
      241 agccgcgact gtgatgcgct aatggcgggc tgcatccagg aggcccgtga gcgatggaac
      301 ttcgactttg tcaccgagac accactggag ggtgacttcg cctgggagcg tgtgcggggc
      361 cttggcctgc ccaagctcta ccttcccacg gggccccggc gaggccggga tgagttggga
      421 ggaggcaggc ggcctggcac ctcacctgct ctgctgcagg ggacagcaga ggaagaccat
      481 gtggacctgt cactgtcttg tacccttgtg cctcgctcag gggagcaggc tgaagggtcc
      541 ccaggtggac ctggagactc tcagggtcga aaacggcggc agaccagcat gacagatttc
      601 taccactcca aacgccggct gatcttctcc aagaggaagc cctaaggatc ccgggtggca
      661 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
      721 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
      781 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga ca
//
