LOCUS       sequence-100211-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Zhou BP, Liao Y, Xia W, Spohn B, Lee MH, Hung MC
  TITLE     Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced 
            phosphorylation in HER-2/neu-overexpressing cells.
  JOURNAL   Nat Cell Biol. 2001 Mar . 3(3):245-52.
  PUBMED    11231573
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2001 Mar . 3(3):245-52."
COMMENT     Sequence Label: Flag p21 WT
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(171..665)
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     CDS             complement(687..710)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             720..742
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccttgctcca taccaccccc ctccacccca taatattata gaaggacacc tagtcagaca
       61 aaatgatgca acttaatttt attaggacaa ggctggtggg cactggagtg gcaacttcca
      121 gggccaggag aggcactggg gaggggtcac agggatgcca cccgggatcc ttagggcttc
      181 ctcttggaga agatcagccg gcgtttggag tggtagaaat ctgtcatgct ggtctgccgc
      241 cgttttcgac cctgagagtc tccaggtcca cctggggacc cttcagcctg ctcccctgag
      301 cgaggcacaa gggtacaaga cagtgacagg tccacatggt cttcctctgc tgtcccctgc
      361 agcagagcag gtgaggtgcc aggccgcctg cctcctccca actcatcccg gcctcgccgg
      421 ggccccgtgg gaaggtagag cttgggcagg ccaaggcccc gcacacgctc ccaggcgaag
      481 tcaccctcca gtggtgtctc ggtgacaaag tcgaagttcc atcgctcacg ggcctcctgg
      541 atgcagcccg ccattagcgc atcacagtcg cggctcagct gctcgctgtc cactgggccg
      601 aagaggcggc ggcaggcctt gctgccgcat gggttctgac ggacatcccc agccggttct
      661 gacatatgca cagatgctct tcttgcttta tcgtcatcgt ctttgtagtc catggtatat
      721 ctccttctta aagttaaaca aaattatttc tagagtcga
//
