LOCUS       sequence-100218-        2117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor targeted to the plasma membrane via Lyn
            kinase targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: PM-ABKAR
FEATURES             Location/Qualifiers
     source          1..2117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(135..137,138..140,141..851)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1374..2108
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgcggccg ccaccatggg ctgcatcaag agcaagcgca
      121 aggacaagga tcccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      181 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      301 tgccctggcc caccctcgtg accaccctga gctggggcgt gcagtgcttc gcccgctacc
      361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      541 acatcctggg gcacaagctg gagtacaacg ccatccacgg caacgtctat atcaccgccg
      601 acaagcagaa gaacggcatc aaggccaact tcggcctcaa ctgcaacatc gaggacggca
      661 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      721 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
      781 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      841 agctgtacaa gcgcatgcat aagttttctc aagaacagat cggcgaaaac attgtgtgca
      901 gggtcatttg taccacgggt caaattccca tccgagattt gtcagctgat atttcacaag
      961 tgcttaagga aaaacgatcc ataaagaaag tttggacatt tggtagaaac ccagcctgtg
     1021 actatcattt aggaaacatt tcaagactgt caaataagca tttccaaata ctactaggag
     1081 aagacggtaa ccttttattg aatgacattt ccactaatgg gacctggtta aatgggcaaa
     1141 aagtcgagaa gaacagcaat cagttactgt ctcaaggtga tgaaataacc gttggtgtag
     1201 gcgtggaatc agatatttta tctctggtca ttttcataaa cgacaaattt aagcagtgcc
     1261 tcgagcagaa caaagttgat cgctctgcag gtaagccagg cagcggcgag ggcagcacca
     1321 agatgcgtcg tgtcgccacc ctggtggacc tcggcaccgg cggcagcgag ctcatgggcg
     1381 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1441 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     1501 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1561 agctgtacaa gggaggtacc ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg
     1621 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1681 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     1741 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggccttcagt
     1801 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1861 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1921 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1981 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     2041 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     2101 acatcgagta agaattc
//
