LOCUS       sequence-100220-        2197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor targeted to the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: ER-ABKAR
FEATURES             Location/Qualifiers
     source          1..2197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(215..217,218..220,221..931)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1454..2188
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc ccttgcagct tccagttgtc aggaagagaa
      121 ggctcccatg gaccctgtgg tggtgctggg gctctgtctc tcctgtttgc ttctcctttc
      181 actctggaaa cagagctatg ggggagggga tcccatggtg agcaagggcg aggagctgtt
      241 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      301 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      361 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gctggggcgt
      421 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      481 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      541 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      601 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ccatccacgg
      661 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcggcctcaa
      721 ctgcaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      781 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      841 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      901 gatcactctc ggcatggacg agctgtacaa gcgcatgcat aagttttctc aagaacagat
      961 cggcgaaaac attgtgtgca gggtcatttg taccacgggt caaattccca tccgagattt
     1021 gtcagctgat atttcacaag tgcttaagga aaaacgatcc ataaagaaag tttggacatt
     1081 tggtagaaac ccagcctgtg actatcattt aggaaacatt tcaagactgt caaataagca
     1141 tttccaaata ctactaggag aagacggtaa ccttttattg aatgacattt ccactaatgg
     1201 gacctggtta aatgggcaaa aagtcgagaa gaacagcaat cagttactgt ctcaaggtga
     1261 tgaaataacc gttggtgtag gcgtggaatc agatatttta tctctggtca ttttcataaa
     1321 cgacaaattt aagcagtgcc tcgagcagaa caaagttgat cgctctgcag gtaagccagg
     1381 cagcggcgag ggcagcacca agatgcgtcg tgtcgccacc ctggtggacc tcggcaccgg
     1441 cggcagcgag ctcatgggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1501 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1561 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1621 gatcactctc ggcatggacg agctgtacaa gggaggtacc ggtggatcta tggtgagcaa
     1681 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1741 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1801 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1861 cctgggctac ggccttcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     1921 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1981 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     2041 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2101 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     2161 caacttcaag atccgccaca acatcgagta agaattc
//
