LOCUS       sequence-100221-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(299..345)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(334..354)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tattctgttc tttcttctag tgtatcttct tctagcagtt ctcttcagtc tagtagcctc
       61 agcagtctct ccggaatcga acagcagagc tccgatcagg ttcttcttga tggagtgtct
      121 atcagtgttt cccagcacct tgaacttctt ggatggcacc ttgtactcat cagtgatcac
      181 agcccatccc acggagttag ttccgatatc cagtccgatg gagtacttct tatccatgtc
      241 gacctggtgg cgcgcctcga gggggggccc ggtacccggg gatcctctag agtcgaggtc
      301 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
      361 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
      421 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
      481 ctgtcggcag aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag
      541 gagccacctt ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat
      601 ccgaggaggt ttccggatat taccc
//
