LOCUS       sequence-100222-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gactaacaga aaggtgactg tgaagcaact gaaggaggat tacttcaaga agatcgagtg
       61 cttcgattcc gtggagatct ccggagtgga ggatagattc aacgcttccc tgggaactta
      121 ccacgatctg ctgaagatca tcaaggataa ggatttcctg gataacgagg agaacgagga
      181 tatcctggag gatatcgtgc tgactctgac tctgttcgag gatagagaga tgatcgagga
      241 gagactgaag acttacgctc acctgttcga tgataaggtg atgaagcaac tgaagagaag
      301 aagatacact ggatggggaa gactgtccag aaagctgatc aacggaatca gagataagca
      361 atccggaaag actatcctgg atttcctgaa gtccgatgga ttcgctaaca gaaacttcat
      421 gcaactgatc cacgatgatt ccctgacttt caaggaggat atccaaaagg ctcaagtgtc
      481 cggacaagga gattccctgc acgagcacat cgctaacctg gctggatccc cagctatcaa
      541 gaagggaatc ctgcaaactg tgaaggtggt ggatgagctg gtgaaggtga tgggaagaca
      601 caagccagag aacatcgtga tcgagatggc tagagagaac caaactactc aaaagggaca
      661 aaaag
//
