LOCUS       sequence-100223-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    31..205
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(212..763)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
       61 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
      121 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
      181 ctactcacac attattatgg agaaactcga gtcaaatctc ggtgacgggc aggaccggac
      241 ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
      301 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
      361 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
      421 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
      481 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc
      541 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
      601 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
      661 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
      721 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagac tcgagagaga
      781 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaact
//
