LOCUS       sequence-100224-         440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     166..188
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     180..197
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     181..197
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca ttcaggctgc
       61 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      121 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      181 gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca
      241 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      301 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      361 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      421 aaggaaaggc catcgttgaa
//
