LOCUS       sequence-100225-        2171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor  targeted to the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: Mito-ABKAR
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(189..191,192..194,195..905)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1428..2162
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 cngagctctc tggctnacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgccacca tggcaatcca gttgcgttcg ctcttcccct
      121 tggcgttgcc cggaatgctg gccctccttg gctggtggtg gtttttctct cgtaaaaaag
      181 cggatcccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      361 ggcccaccct cgtgaccacc ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc
      421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      601 tggggcacaa gctggagtac aacgccatcc acggcaacgt ctatatcacc gccgacaagc
      661 agaagaacgg catcaaggcc aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc
      721 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      781 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      841 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      901 acaagcgcat gcataagttt tctcaagaac agatcggcga aaacattgtg tgcagggtca
      961 tttgtaccac gggtcaaatt cccatccgag atttgtcagc tgatatttca caagtgctta
     1021 aggaaaaacg atccataaag aaagtttgga catttggtag aaacccagcc tgtgactatc
     1081 atttaggaaa catttcaaga ctgtcaaata agcatttcca aatactacta ggagaagacg
     1141 gtaacctttt attgaatgac atttccacta atgggacctg gttaaatggg caaaaagtcg
     1201 agaagaacag caatcagtta ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg
     1261 aatcagatat tttatctctg gtcattttca taaacgacaa atttaagcag tgcctcgagc
     1321 agaacaaagt tgatcgctct gcaggtaagc caggcagcgg cgagggcagc accaagatgc
     1381 gtcgtgtcgc caccctggtg gacctcggca ccggcggcag cgagctcatg ggcggcgtgc
     1441 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1501 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     1561 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1621 acaagggagg taccggtgga tctatggtga gcaagggcga ggagctgttc accggggtgg
     1681 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1741 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1801 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctt cagtgcttcg
     1861 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1921 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1981 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2041 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     2101 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     2161 agtaagaatt c
//
