LOCUS       sequence-100226-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with bar plant 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC123
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..77)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             163..171
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(224..235)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tcacgcgggg cggccgctct agaactagtt aattaatctg gtctagatca tcctcccacc
       61 tttctcttct tctttggttg cagatctcct cccagttggg acagatcgat tctagtctcg
      121 tacagtccag tgatggattg gtggatcaga gtagcatcca gcacctcctt agtggaagtg
      181 tatctctttc tatcgatagt agtatcgaag tacttgaaag cagctggagc tcccaggtta
      241 gtcagagtga acaggtggat gatgttctca gcttgctctc tgattggctt atctctgtgc
      301 ttgttgtaag cggacagcac cttatccagg ttagcatcag ccaggatcac tctcttggag
      361 aactcggaga tttgctcgat gatctcatcc aggtagtgct tgtgttgctc cacgaacagt
      421 tgcttttgct cgttatcctc tggggatccc ttcagcttct cgtagtggga agccaggtac
      481 aggaagttca cgtacttgga tggcagagcc agctcgtttc ccttttgcag ctctccagcg
      541 gaagccagca ttctctttct tccgttctcc agctcgaaca gggagtactt tggcagcttg
      601 atgatcagat ccttcttcac ctccttgtat cccttagcct ccaggaaatc gattgggttc
      661 ttctcgaagg aggatctctc catgatagtg attcccagca gctccttcac ggacttcagc
      721 ttcttggact ttcccttctc caccttagcc accaccagca
//
